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Literature summary extracted from

  • Xi, J.H.; Bai, F.; McGaha, R.; Andley, U.P.
    Alpha-crystallin expression affects microtubule assembly and prevents their aggregation (2006), FASEB J., 20, 846-857.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.5.6 tubulin unassembled tubulin denatures relatively easily, and the denatured tubulin can inhibit the assembly itself Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.5.6 49800
-
 theoretical molecular mass, beta tubulin Mus musculus
3.6.5.6 49800
-
 theoretical molecular mass, beta tubulin Bos taurus
3.6.5.6 50000
-
 alpha-beta tubulin Bos taurus
3.6.5.6 50000
-
 determined by SDS-PAGE and immunoblot analysis Mus musculus
3.6.5.6 50130
-
 theoretical molecular mass, alpha tubulin Mus musculus
3.6.5.6 50130
-
 theoretical molecular mass, alpha tubulin Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.5.6 GTP + H2O Mus musculus
-
 GDP + phosphate
-
 ir
3.6.5.6 GTP + H2O Bos taurus
-
 GDP + phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.6 Bos taurus
-
-
-
3.6.5.6 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.5.6 microtubules are prepared from freshly isolated lens epithelial fractions from mice Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.5.6 brain
-
 Bos taurus
-
3.6.5.6 lens lens epithelial cell Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.6 GTP + H2O
-
 Mus musculus GDP + phosphate
-
 ir
3.6.5.6 GTP + H2O
-
 Bos taurus GDP + phosphate
-
 ir

Subunits

EC Number Subunits Comment Organism
3.6.5.6 heterodimer
-
 Mus musculus
3.6.5.6 heterodimer
-
 Bos taurus
3.6.5.6 More elongated microtubule protofilaments form at 37°C in the presence of GTP and later close into hollow cylindrical polymers Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.6.5.6 tubulin GTPase
-
 Mus musculus
3.6.5.6 tubulin GTPase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.5.6 37
-
 elongated microtubule protofilaments form at 37°C in the presence of GTP and later close into hollow cylindrical polymers Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.5.6 0.0077
-
 GTP in the presence of alphaA-crystallin Bos taurus
3.6.5.6 0.008
-
 GTP in the presence of alphaB-crystallin Bos taurus
3.6.5.6 0.0083
-
 GTP
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.5.6 6.8
-
 assembly of microtubules Mus musculus